List of Publications


  • Z. Chorghay, D. MacFarquhar, V. J. Li, S. Aufmkolk, A. Schohl, P. W. Wiseman, R. T. Káradóttir, E. S. Ruthazer. Activity-dependent alteration of early myelin ensheathment in a developing sensory circuit. Journal of Comparative Neurology Accepted 9/28/2021

  • JS. Sehayek, X. Yi, S. Weiss, P. W. Wiseman. Rapid ensemble measurement of protein diffusion, probe blinking and photobleaching dynamics in the complex cellular space. Biophysical Reports 1:100015 (2021)

  • K. Kolosova, Q. Gao, M. Tuznik, S. Bouhabel, K. M. Kost, H. Wang, N. Y. K. Li-Jessen, L. Mongeau, P. W. Wiseman. Characterizing vocal fold injury recovery in a rabbit model with 3D virtual histology. The Laryngoscope 131:1578-1587 (2021)

  • A. A. Gopal, A. Kazarine, J. M. Dubach, P. W. Wiseman. Recent Advances in Nonlinear Microscopy: Deep Insights and Polarized Revelations. International Journal of Biochemistry and Cell Biology 130:105896 (2021)

  • J. L. Kure, C. B. Andersen, K. I. Mortensen, P. W. Wiseman, E. C. Arnspang. Revealing plasma membrane nano-domains with diffusion analysis methods. Membranes 10(11):314 (2020)

  • Y. Qian, D M. O. Cosio2, K. D. Piatkevich, S. Aufmkolk, W.-C. Su, O. T. Celiker, A. Schohl, M. H. Murdock, A. Aggarwal, Y.-F. Chang, P. W. Wiseman, E. S. Ruthazer, E. S. Boyden, R. E. Campbel. Improved genetically encoded near-infrared fluorescent calcium ion indicators for in vivo imaging. PLOS Biology 18(11):33000965 (2020)

  • C. Schneider, C. Shen A. A. Gopal, T. Douglas, B. Forestell, K. D. Kauffman, P. Artusa, D. Rogers, Q. Zhang, H. Jing, A. F. Freeman, D. L. Barber, M. Saleh, P. W. Wiseman, H. C. Su, J. N. Mandl. Migration-induced cell shattering due to DOCK8-deficiency causes a type-2-biased helper T cell response. Nature Immunology 21:1528-1539 (2020)

  • D. J. Foust, A. G. Godin, A. Ustione, P. W. Wiseman, D. W. Piston. Two-color spatial cumulant analysis detects heteromeric interactions between membrane proteins. Biophysical Journal 117(9):1764-1777 (2019)

  • S. Sehayek, Y. Gidi, V. Glembockyte, H. B. Brandao, P. Francois, G. Cosa, P. W. Wiseman. A high-throughput image correlation method for rapid analysis of fluorophore photo-blinking and bleaching rates. ACS Nano 13(10):1764-1777 (2019)

  • K. van den Dries, L. Nahidiazar, J. A. Slotman, M. B. M. Meddens, E. Pandzic, B. Joosten, M. Ansems, J. Schouwstra, A. Meijer, R. Steen, M. Weijers, J. Fransen, A. B. Houtsmuller, P. W. Wiseman, K. Jalink, and A. Cambi. Modular actin nano-architecture enables podosome protrusion and mechanosensing. Nat. Commun. 10:5171 (2019)

  • N. Mikolajewicz, S. Sehayek, P. W. Wiseman, S. V. Komarova. Transmission of mechanical information by purinergic signaling. Cell Reports 116(10):2009-2022 (2019)

  • A. I. Makarkov, M. Golizeh, E. Ruiz-Lancheros, A. A. Gopal, I. N. Costas-Cancelas, S. Chierzi, S. Pillet, N. Charland, N. Landry, I. Rouiller, P. W. Wiseman, M. Ndao, B. J. Ward. Plant-derived virus-like particle vaccines drive cross-presentation of influenza A hemagglutinin peptides by human monocyte-derived macrophages. NPJ Vaccines 4:17 (2019)

  • A. Kazarine, A. A. Gopal, P. W. Wiseman. Nonlinear microscopy of common histological stains reveals THG harmonophores. Analyst 144:3239-3249 (2019)

  • A. Abu-arish, E. Pandzic, D. Kim, H. W. Tseng, P. W. Wiseman and J. W. Hanrahan. Agents that stimulate secretion promote CFTR recruitment into membrane lipid microdomains. Journal of General Physiology 151(6):834-849 (2019)

  • A. Kazarine, K. Kolosova, A. A. Gopal, H. Wang, R. Tahara, A. Rammal, K. Kost, L. Mongeau, N. Y. K. Li-Jessen, P. W. Wiseman. Multimodal virtual histology of rabbit vocal folds by nonlinear microscopy and nano-computed tomography. Biomed. Opt. Express 10(3):1151-1164 (2019)

  • A. Kazarine, F. Baakdah, A. A. Gopal, W. Oyibo, E. Georges, P. W. Wiseman. Malaria detection by third harmonic generation image scanning cytometry. Analytical Chemistry 91(3):2216-2223 (2019)

  • S. D. Glasgow, S. Labrecque, I. V. Beamish, S. Aufmkolk, J. Gibon, D. Han, S. N. Harris, P. Dufresne, P. W. Wiseman, R. A. McKinney, P. Séguélal, P. De Koninck, E. S. Ruthazer, T. E. Kennedy. Activity-dependent netrin-1 secretion drives synaptic insertion of GluA1-containing AMPA receptors in the hippocampus. Cell Reports 25(1):168-182 (2018)

  • G. M. Fortin, C. Ducrot, N. Giguère, Willemieke M. Kouwenhoven, M.-J. Bourque, C. Pacelli, R. K. Varaschin, M. Brill, S. Singh, P. W. Wiseman, L.-É. Trudeau. Segregation of dopamine and glutamate release sites by dopamine neurons is regulated by striatal neurons. The FASEB Joural 33(1):400-417 (2018)

  • E. Pandzic, A. Abu-Arish, R. W. Whan, J. W. Hanrahan, P. W. Wiseman. Velocity landscape correlation resolves multiple flowing protein populations from fluorescence image time series. Methods 140-141:126-139 (2018)

  • T. Toplak, B. Palmieri, A. Juanes-Garcia, M. Vicente-Manzanares, M. Grant, P. W. Wiseman. WIMS Reveals Focal Adhesion Distributions, Dynamics and Coupling between Actomyosin Bundle Stability. PLOS ONE 12(10):e0186058 (2017)

  • A. A. Hariri, G. D. Hamblin, J. S. Hardwick, R. Godin, J.F. Desjardins, P. W. Wiseman, H. F. Sleiman, G. Cosa. Stoichiometry and dispersity of DNA nanostructures using photobleaching pair-correlation analysis. Bioconjugate Chemistry 28:2340-2349 (2017)

  • A. A. Gopal, S. G. Ricoult, S. N. Harris, D. Juncker, T. E. Kennedy, P. W. Wiseman. Spatially selective dissection of signal transduction in neurons on netrin-1 printed nanoarrays via segmented fluorescence fluctuation analysis. ACS Nano 11:8131-8143 (2017)

  • C. Triplet van Oostende, D. Guillet, T. Triplet, E. Pandzic, P. W. Wiseman, A. Geitmann. Vesicle dynamics during plant cell cytokinesis reveals distinct functional phases. Plant Physiology 174(3):1544-1558 (2017)

  • G. W. Ashdown, G. L. Burn, D. J. Williamson, E. Pandzic, R. Peters, M. Holden, H. Ewers, L. Shao, P. W. Wiseman, D. M. Owen. Live-cell super-resolution reveals F-actin and plasma membrane dynamics at the T cell synapse. Biophys. J. 112:1703–1713, (2017)

  • V. Rouger, R. Alchini, A. Kazarine, A. A. Gopal, M.-P. Girouard, A. E. Fournier, P. W. Wiseman. Low cost multi-modal light sheet microscopy for optically cleared tissues and living specimens. Journal of Biomedical Optics 21(12):126008 (2016)

  • M. B. Meddens, E. Pandzic, J. A. Slotman, D. Guillet, B. Joosten, S. Mennens, L. M. Paardekooper, A. B. Houtsmuller, K. van den Dries, P. W. Wiseman, A. Cambi. Actomyosin-dependent dynamic spatial patterns of cytoskeletal components drive mesoscale podosome organization. Nat. Comm. 7:13127-13143 (2016)
    F1000Prime Recommendation

  • L.S. Miraucourt, J. Tsui, D. Gobert, J.-F. Desjardins, A. Schohl, M. Sild, P. Spratt, A. Castonguay, Y. De Koninck, N. Marsh-Armstrong, P.W. Wiseman, E.S. Ruthazer. Endocannabinoid signaling enhances visual responses through modulation of intracellular chloride levels in retinal ganglion cells. eLife 5:e15932 (2016)

  • Y. Arata, M. Hiroshima, C.-G. Pack, R. Ramanujam, F. Motegi, K. Nakazato, Y. Shindo, P. W. Wiseman, H. Sawa, T. J. Kobayashi, H. B. Brandão, T. Shibata, Y. Sako. Cortical Polarity of the RING Protein PAR-2 is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary. Cell Rep. 16(8):2156–2168 (2016)

  • B. Rappaz, K. Lai Wing Sun, J. P. Correia, P. W. Wiseman, T. E. Kennedy. FLIM FRET visualization of Cdc42 activation by netrin-1 in embryonic spinal commissural neuron growth cones. PLoS ONE 11(8):e0159405 (2016)

  • H. Klein, A. Abu-Arish, N. T. N. Trinh, Y. Luo, P. W. Wiseman, J. W. Hanrahan, E. Brochiero, R. Sauvé. Investigating CFTR and KCa3.1 Protein/Protein Interactions. PLoS One 11(4):e0153665 (2016)

  • A. A. Gopal, B. Rappaz, I. B. Martyn, P. D. Dahlberg, R. J. Meland, I. V. Beamish, T. E. Kennedy, P. W. Wiseman. Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS. Biophys. J. 110:623-634 (2016)

  • G. Ashdown, E. Pandzic, A. Cope, P. Wiseman, D. Owen. Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data. JoVE 106 (2015)

  • A. G. Godin, B. Rappaz, L. Potvin-Trottier, T. E. Kennedy, Y. DeKoninck, P. W. Wiseman. Spatial intensity distribution analysis reveals abnormal oligomerization of proteins in single cells. Biophys. J. 109:710-721 (2015) New and Notable selection

  • A. Abu-Arish, E. Pandzic, J. Goeppe, E. Matthes, J. W Hanrahan, P. W. Wiseman. Cholesterol modulates CFTR confinement in the plasma membrane of primary epithelial cells. Biophys. J. 109:85-94 (2015) New and Notable selection

  • H. K. Heris, A. K. Miri, N. R. Ghattamaneni, N. Y.K. Li, S. L. Thibeault, P. W. Wiseman, L. Mongeau. Microstructural and mechanical characterization of scarred vocal folds. J. Biomechanics 48:708-711 (2015)

  • G. W. Ashdown, A. Cope, P. W. Wiseman, D. M. Owen. Molecular flow quantified beyond the diffraction limit by spatio-temporal image correlation of structured illumination microscopy data. Biophys. J. Lett. 107:L21–L23 (2014)

  • M. R. Khazaei, M.-P. Girouard, R. Alchini, S. O. Tone, T. Shimada, S. Bechstedt, M. Cowan, D. Guillet, P. W. Wiseman, G. Brouhard, J. F. Cloutier, A. E. Fournier. Collapsin response mediator protein 4 regulates growth cone dynamics through the actin and microtubule cytoskeleton. J. Biol. Chem. 289: 30133-30143 (2014)

  • L.E. Lorenzo, A. G. Godin, F. Wang, M. St-Louis, S. Carbonetto, P. W. Wiseman, A. Ribeiro-da-Silva and Y. De Koninck. Gephyrin Clusters Are Absent from Small Diameter Primary Afferent Terminals Despite the Presence of GABAA Receptors. J. Neurosci. 34: 8300-8317 (2014)

  • K. E. Bujold, J. Fakhoury, T. G. W. Edwardson, K. M. M. Carneiro, J. N. Briard, A. G. Godin, L. Amrein, G. D. Hamblin, L. C. Panasci, P. W. Wiseman and H. F. Sleiman. Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles. Chem. Sci. 5: 2449-2455 (2014)

  • N. Jowett, W. Wöllmer, R. Reimer, J. Zustin, U. Schumacher, P. Wiseman, A. Mlynarek, A. Böttcher, C. Dalchow, B. Lörincz, R. Knecht, and R. J. D. Miller. Bone Ablation without Thermal or Acoustic Mechanical Injury via a Novel Picosecond Infrared Laser (PIRL). JAMA Otolaryngology - Head and Neck Surgery 150: 385-393 (2014)

  • H. B. Brandao, H. Sangji, E. Pandzic, S. Bechstedt, G. J. Brouhard, P. W. Wiseman. Measuring ligand-receptor binding kinetics and dynamics using k-space image correlation spectroscopy. Methods 66: 273-282 (2014)
    Invited Paper: Special issue on Advanced Light Microscopy Imaging in Biological Sciences.
    F1000Prime Recommendation

  • E. C. Arnspang, J. S. Koffman, S. Marlar, P. W. Wiseman, L. N. Nejsum. Easy measurement of diffusion coefficients of EGFP-tagged plasma membrane proteins using a MatLab code for k-space Image Correlation Spectroscopy. JoVE 87 (2014)

  • E. C. Arnspang, J. Schwartzentruber, M. P. Clausen, C. Lyngsø, J. L. Hansen, P. W. Wiseman, B. C. Lagerholm. Bridging the gap between single molecule and ensemble methods for measuring lateral dynamics in the plasma membrane. PLoS ONE 8(12): e78096 (2013)

  • P. W. Wiseman. Fluctuation Imaging Spiced Up with a Piece of PIE. Biophys. J. 105: 831 (2013)

  • A. Barbeau, J. L. Swift, A. G. Godin, Y. De Koninck, P.W. Wiseman, J.-M. Beaulieu. Spatial Intensity Distribution Analysis (SpiDA), a new tool for receptor tyrosine kinase activation and transactivation quantification. Methods in Cell Bio. 117: 1-19 (2013)

  • L. Potvin-Trottier, L. Chen, A. R. Horwitz, P. W. Wiseman. A nu-space for ICS: characterization and application to measure protein transport in live cells. New J. Phys. 15: 085006 (2013). Focus on Soft Mesoscopics: Physics for Biology at a Mesoscopic Scale

  • K. Hossein Heris, A. K. Miri, U. Tripathy, P. W. Wiseman, L. Mongeau. Microstructural Characterization of Vocal Folds toward a Strain-Energy Formulation. Acta Biomater. 9(8): 7957-7967 (2013)

  • U. Tripathy, M. Giguère-Bisson, M. H. Sangji, M.-J. Bellemare, D. S. Bohle, E. George, P. W. Wiseman. Optimization of the third harmonic generation response for imaging detection of the malaria pigment hemozoin. Anal. Bioanal. Chem. 405: 5431-5440 (2013)

  • P. W. Wiseman. Image correlation spectroscopy: mapping correlations in space, time, and reciprocal space. Meth. Enzymol. 518: 245-267 (2013)

  • B. Rappaz, P. W. Wiseman. Image Correlation Spectroscopy for measurements of particle densities and colocalization. Curr. Protoc. Cell Bio. 4.27.1-4.27.15 (2013)

  • N. Jowett, W. Woellmer, A. M. Mlynarek, P. Wiseman, B. Segal, K. Franjic, P. Kroetz, A. Boettcher, R. Knecht, R. J. D. Miller. Heat generation during ablation of porcine skin with Er:YAG laser versus a novel picosecond infrared laser (PIRL). JAMA Otolaryngology — Head & Neck Surgery 139(8): 828-833 (2013)

  • A. Barbeau, A. G. Godin, J. L. Swift, Y. De Koninck, P. W. Wiseman, and J.-M.Beaulieu. Quantification of receptor tyrosine kinase activation and transactivation by G Protein Coupled receptors using Spatial Intensity Distribution Analysis (SpIDA). Meth. Enzymol. 522: 109-131 (2013)

  • T. Toplak, E. Pandzic, L. Chen, M. Vicente-Manzanares, A. R. Horwitz, P. W. Wiseman. STICCS Reveals Matrix-Dependent Adhesion Slipping and Gripping in Migrating Cells. Biophys. J. 103(8):1672-1682 (2012)

  • L. Chen, M. Vicente-Manzanares, L. Potvin-Trottier, P. W. Wiseman, A. R. Horwitz. The integrin-ligand interaction regulates adhesion and migration through a molecular clutch. PLoS ONE 7(7):1-15 (2012)

  • M. Sergeev, A. G. Godin, L. Kao, N. Abuladze, P. W. Wiseman, I. Kurtz. Determination of Membrane Protein Transporter Oligomerization in Native Tissue Using Spatial Fluorescence Intensity Fluctuation Analysis. PLoS ONE 7(4):1-11 (2012)

  • M. Sergeev, J. L. Swift, A. G. Godin, P. W. Wiseman. Ligand-induced clustering of EGF receptors : A quantitative study by fluorescence image moment analysis. Biophys. Chem. 161:50-53 (2012)

  • A. K. Miri, U. Tripathy, L. Mongeau, P. W. Wiseman. Nonlinear Laser Scanning Microscopy of Human Vocal Folds. The Laryngoscope 122(2):356-363 (2012)

  • N. Durisic, A. Godin, D. Walters, P. Grutter, P. W. Wiseman, C. Heyes. Probing the “dark” fraction of core-shell quantum dots by ensemble and single particle pH-dependent spectroscopy. ACS Nano 5(11):9062-9073 (2011)

  • S. Boyle, D. Kolin, J. G. Bieler, J. P. Schneck , P. W. Wiseman, M. Edidin. Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen. Biophys. J. 101:L57-L59 (2011)

  • H. Balghi, R. Robert, B. Rappaz, X. Zhang, A. Wohlhuter-Haddad, A. Evagelidis, Y. Luo, J. Goepp, P. Ferraro, P. Roméo, M. Trebak, P. W. Wiseman, D. Y. Thomas, J. W. Hanrahan. Enhanced Ca2+ entry due to Orai1 plasma membrane insertion increases IL-8 secretion by cystic fibrosis airways. FASEB J. 25(12):4274-4291 (2011)

  • B. Mesnage, S. Gaillard, A. Godin, J.-L. Rodeau, M. Hammer, J. von Engelhardt, P. W. Wiseman, Y. De Koninck, R. Schlichter, M. Cordero-Erausquin. Morphological and functional characterization of cholinergic interneurons in the dorsal horn of the mouse spinal cord. Journal of Comparative Neurology 519:3139-3158 (2011)

  • J. L. Swift, A. G. Godin, K. Doré, L. Freland, N. Bouchard, C. Nimmo, M. Sergeev, Y. De Koninck, P. W. Wiseman, J. M. Beaulieu. Quantification of receptor tyrosine kinase transactivation through direct dimerization and surface density measurements in single cells. Proc. Natl. Acad. Sci. U.S.A. 108(17): 7016-7021 (2011)

  • A. G. Godin, S. Costantino, L. E. Lorenzo, J. L. Swift, M. Sergeev, A. Ribeiro-da-Silva, Y. De Koninck, P. W. Wiseman. Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis. Proc. Natl. Acad. Sci. U.S.A. 108(17): 7010-7015(2011)

  • V. Baumgärtel, S. Ivanchenko, A. Dupont, M. Sergeev, P. W. Wiseman, H-G Kräusslich, C. Bräuchle, B. Müller, D. C. Lamb Dynamics of HIV budding site interactions with an ESCRT component visualized in live-cells. Nature Cell Biology 13:469–474 (2011)

  • J. L. Swift, M. Sergeev, P.W. Wiseman. Fluorescence microscopy investigations of ligand propagation and accessibility under adherent cells. Biointerfaces 5(4): (2010)

  • P. Opazo, S. Labrecque, C. M. Tigaret, A. Frouin, P. W. Wiseman, P. De Koninck, D. Choquet. CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of Stargazin. Neuron 67(2):239-252 (2010)

  • J. M. Belisle, T. Zigras, S. Costantino, R. Cartier, J. Butany, P. W. Wiseman, R. L. Leask. Second Harmonic Generation Microscopy to Investigate Collagen Configuration: A Pericarditis Case Study. Cardiovascular Pathology 19(4):e125-e128 (2009)

  • N. Durisic, P. W. Wiseman, P. Grutter, C. Heyes. A common mechanism underlies the dark fraction formation and fluorescence blinking of quantum dots. ACS Nano 3(5): 1167-1175 (2009)

  • K. G. Heinze, S. Costantino, P. De Koninck, P. W. Wiseman. Beyond photobleaching, laser illumination unbinds fluorescent proteins. J Phys Chem B. 113(15): 5225-33(2009)

  • M. A. Digman, P. W. Wiseman, C. Choi, A. R. Horwitz and E. Gratton. Stoichiometry of molecular complexes at adhesions in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (7): 2170-2175 (2009)

  • M. A. Digman, P. W. Wiseman, A. R. Horwitz, E. Gratton. Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method. Biophys. J. 96 (2): 707-716 (2009)
    Faculty of 1000 Biology Selection

  • J. M. Bélisle, J. P. Correia, P. W. Wiseman, T. E. Kennedy, S. Costantino. Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP. Lab on a Chip. 147: DOI: 10.1039/b813897d (2008) Highlighted in Chemical Technology October 30, 2008

  • J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, A. Geitmann. Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy (STICS) and fluorescence recovery after photobleaching (FRAP). Plant Physiology. 147: 1646-1658 (2008)

  • P. J. Darlington, C. Podjaski, K. E. Horn, S. Costantino, M. Blain, P. Saikali, Z. Chen, K. A. Baker, J. Newcombe, M. Freedman, P. W. Wiseman, A. Bar-Or, T. E. Kennedy, J. P. Antel. Innate Immune-mediated Neuronal Injury Consequent to Loss of Astrocytes. Journal of Neuropathology and Experimental Neurology. 67(6): 590-599 (2008)

  • J. W. D. Comeau, D. L. Kolin, P. W. Wiseman. Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy. Molecular Biosystems. 4: 672 - 685 (2008)
    Invited paper "New Emerging Investigators special issue"

  • A. I. Bachir, D. L. Kolin, K. G. Heinze, B. Hebert, P. W. Wiseman. A guide to accurate measurement of diffusion using fluorescence correlation techniques with blinking quantum dot nanoparticle labels. J. Chem. Phys. 128: 225105-1-225105-11 (2008)

  • S. Costantino, C. B. Kent, A. G. Godin, T. E. Kennedy, P. W. Wiseman, A. E. Fournier. Semi-automated quantification of filopodial dynamics. Journal of Neuroscience Methods. 171: 165-173 (2008)

  • J. M. Belisle, S. Costantino, M. L. Leimanis, M.-J. Bellemare, D. S. Bohle, E. Georges, P. W. Wiseman. Sensitive Detection of Malaria Infection by Third Harmonic Generation Imaging. Biophys. J.: Biophysical Letter 94:L26-L28 (2008) Highlighted in Biophotonics International, February 2008; Highlighted in BioOptics World March 2008; Highlighted in MalariaWorld; Highlighted in Analytical Chemistry Online News October 30, 2008.

  • R. Khatchadourian, A. I. Bachir, S. J. Clarke, C. D. Heyes, P. W. Wiseman, J. L. Nadeau. Fluorescence intensity and intermittency as tools for tracking. Biomed.Biotech. 70145:1-10 (2008)

  • S. Costantino, C. G. McQuinn, T. E. Kennedy, P. W. Wiseman. Fabrication of protein gradients for cell culture using a miniature squeegee. Journal of Biochemical and Biophysical Methods 70: 1192-1195 (2008)

  • P. T. Yam, C. A. Wilson, L. Ji, B. Hebert, E. L. Barnhart, N. A. Dye, P. W. Wiseman, G. Danuser, J. A. Theriot. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. Journal of Cell Biology 178:1207-1221 (2007)

  • D. L. Kolin, P. W. Wiseman. Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently-labeled macromolecules in cells. Cell Biochem. Biophys. 49: 141-164 (2007)

  • N. Durisic, A. I. Bachir, D. L. Kolin, B. Hebert, B. C. Lagerholm, P. Grutter, P. W. Wiseman. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules. Biophys. J. 93: 1338-1346 (2007)

  • D. Fessart, M. Simaan, B. Zimmerman, J. Comeau, F. F. Hamdan, P. W. Wiseman, M. Bouvier, S. A. Laporte. SRC-dependent phosphorylation of beta2-adaptin dissociated the beta-arrestin-AP-2 complex. Journal of Cell Science 120: 1723-1732 (2007)

  • J. W. D. Comeau, S. Costantino, P. W. Wiseman. Measuring colocalization within fluorescence microscopy images. Biophotonics International 14 (3):42-45 (2007)

  • C. M. Brown, B. Hebert, D. L. Kolin, J. Zareno, L. Whitmore, A. R. Horwitz, P. W. Wiseman. Probing the integrin-actin linkage using high-resolution protein velocity mapping. Journal of Cell Science 119:5204-5214 (2006)
    Faculty of 1000 Biology Selection



  • I. R. Bates, P. W. Wiseman, J. W. Hanrahan. Investigating membrane protein dynamics in living cells. Biochemistry & Cell Biology 84:825-831 (2006) Invited Review

  • J. W. D. Comeau, S. Costantino, P. W. Wiseman. A Guide to Accurate Fluorescence Microscopy Colocalization Measurements. Biophys. J. 91:4611-4622 (2006)

  • M. Sergeev, S. Costantino, P. W. Wiseman. Measurement of Monomer-Oligomer Distributions via Fluorescence Moment Image Analysis." Biophys. J. 91:3884-3896 (2006)

  • D. L. Kolin, D. Ronis, P. W. Wiseman. k-Space image correlation spectroscopy (kICS): A method for accurate transport measurements independent of fluorophore photophysics. Biophys. J. 91: 3061-3075 (2006)

  • I. R. Bates, B. Hebert, Y. Luo, J. Liao, A. I. Bachir, D. L. Kolin, P. W. Wiseman, J. W. Hanrahan. Membrane lateral diffusion and capture of CFTR within transient confinement zones. Biophys. J. 91(3):1046-1058 (2006)
    Faculty of 1000 Biology Selection

  • A. Bachir, N. Durisic, B. Hebert, P. Grutter, P. W. Wiseman. Characterization of Blinking Dynamics in Quantum Dot Ensembles using Image Correlation Spectroscopy. J. Appl. Phys. 99: 064503-1-064503-7 (2006). Article selected for March 27, 2006 issue of Virtual Journal of Nanoscale Science & Technology and also selected for the April 1, 2006 issue of Virtual Journal of Biological Physics Research (editorial selection of the most important research papers of the month).

  • D. L. Kolin, S. Costantino, and P. W. Wiseman. Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy. Biophy. J. 90(2):628-639 (2006).

  • S. Costantino, K. G. Heinze, O. E. Martinez, P. De Koninck, P. W. Wiseman. Two-photon fluorescent micro-lithography for live cell imaging Microscopy Research and Technique 68 : 272-276 (2005)

  • S. Costantino, J. W. D. Comeau, D. L. Kolin, P. W. Wiseman. Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy Biophys. J. 89 : 1251-1260 (2005).

  • B. Hebert, S. E. Hulme, P. W. Wiseman. Membrane protein dynamics measured by two-photon ring correlation spectroscopy: theory and application to living cells. Proc. SPIE 5700: 109-117 (2005). Invited Paper

  • M. A. Digman, C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, E. Gratton, Measuring fast dynamics in solutions and cells with a laser scanning microscope, Biophys. J. 89 : 1317-1327 (2005)
    Faculty of 1000 Biology Selection

  • M.A. Digman, P. Sengupta, P.W. Wiseman, C.M. Brown, A.R. Horwitz, E. Gratton. Fluctuation correlation spectroscopy with laser scanning microscope: exploiting the hidden time structure. Biophys. J. 88 : L33-36 (2005).

  • B. Hebert, S. Constantino, P. W. Wiseman. Spatio-temporal Image Correlation Spectroscopy (SITCS): Theory, verification and application to protein velocity mapping in living CHO cells. Biophys. J. 88, 3601-3614 (2005).

  • P. W. Wiseman, C.M. Brown, D.J. Webb, B. Hebert, N.L.Johnson, J.A. Squier, M.H. Ellisman, A.R. Horwitz. Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy, J. Cell Sci. 117, 5521-5534 (2004) Highlighted in Nature Reviews Molecular Cell Biology.
    Faculty of 1000 Biology Selection


  • J. V. Rocheleau, P. W. Wiseman, N. O. Petersen, Isolation of bright aggregate fluctuations in a multipopulation image correlation spectroscopy system using intensity subtraction, Biophys. J. 84, 4011-4022 (2003).

  • P. W. Wiseman, J. A. Squier, Measurement of cell surface protein dynamics by two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4633, 74-83(2002).

  • P. W. Wiseman, J. A. Squier, Live cell studies of adhesion receptors by two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4620, 54-61 (2002).

  • P. W. Wiseman, F. Capani, J. A. Squier, and M. E. Martone, Counting dendritic spines in cerebellar tissue slice by image correlation spectroscopy analysis, J. Microsc. 205, 177-186 (2002).

  • P. W. Wiseman and J. A. Squier, Two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4262, 279-286 (2001).

  • D. N. Fittinghoff, P. W. Wiseman, J. A. Squier, Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy, Opt. Exp. 7, 273-279 (2000).

  • P. W. Wiseman, J. A. Squier, M. H. Ellisman, and K. R. Wilson, Two-photon video rate image correlation spectroscopy (ICS) and image cross-correlation spectroscopy (ICCS), J. Microscopy 200, 14-25 (2000).

  • P. W. Wiseman, J. A. Squier, K. R. Wilson, Dynamic image correlation spectroscopy (ICS) and two-color image cross-correlation spectroscopy (ICCS): concepts and application, Proc. SPIE 3919, 14-20 (2000).

  • A. C. Millard, P. W. Wiseman, D. N. Fittinghoff, K. R. Wilson, J. A. Squier, and M. Müller, Third harmonic generation microscopy using a compact, femtosecond fiber laser source, App. Opt. 38, 7393-7397 (1999).

  • P. W. Wiseman and N. O. Petersen, Image correlation spectroscopy (ICS) II: optimization for ultrasensitive detection of pre-existing PDGF-b receptor oligomers on intact cells, Biophys. J. 76, 963-977 (1999).

  • N. O. Petersen, C. M. Brown, M. Srivastava, J. Rocheleau, P. W. Wiseman, Analysis of membrane protein cluster densities and sizes by in situ image correlation spectroscopy, Faraday Discuss. 111, 289-305 (1998).

  • P. W. Wiseman, P. Höddelius, N. O. Petersen, and K.-E. Magnusson, Aggregation of PDGF-b receptors in human skin fibroblasts: characterization by image correlation spectroscopy (ICS), FEBS Lett. 401, 43-48 (1997).

  • N. O. Petersen, P. L. Höddelius, P. W. Wiseman, O. Seger, and K.-E. Magnusson, Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application, Biophys. J. 65, 1135-1146 (1993).

    •