List of Publications


  • H. Klein, A. Abu-Arish, N. T. N. Trinh, Y. Luo, P. W. Wiseman, J. W. Hanrahan, E. Brochiero, R. Sauvé. Investigating CFTR and KCa3.1 Protein/Protein Interactions. PLoS One 11(4):e0153665 (2016)

  • A. A. Gopal, B. Rappaz, I. B. Martyn, P. D. Dahlberg, R. J. Meland, I. V. Beamish, T. E. Kennedy, P. W. Wiseman. Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS. Biophys. J. 110:623-634 (2016)

  • G. Ashdown, E. Pandzic, A. Cope, P. Wiseman, D. Owen. Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data. JoVE 106 (2015)

  • A. G. Godin, B. Rappaz, L. Potvin-Trottier, T. E. Kennedy, Y. DeKoninck, P. W. Wiseman. Spatial intensity distribution analysis reveals abnormal oligomerization of proteins in single cells. Biophys. J. 109:710-721 (2015) New and Notable selection

  • A. Abu-Arish, E. Pandzic, J. Goeppe, E. Matthes, J. W Hanrahan, P. W. Wiseman. Cholesterol modulates CFTR confinement in the plasma membrane of primary epithelial cells. Biophys. J. 109:85-94 (2015) New and Notable selection

  • H. K. Heris, A. K. Miri, N. R. Ghattamaneni, N. Y.K. Li, S. L. Thibeault, P. W. Wiseman, L. Mongeau. Microstructural and mechanical characterization of scarred vocal folds. J. Biomechanics 48:708-711 (2015)

  • G. W. Ashdown, A. Cope, P. W. Wiseman, D. M. Owen. Molecular flow quantified beyond the diffraction limit by spatio-temporal image correlation of structured illumination microscopy data. Biophys. J. Lett. 107:L21–L23 (2014)

  • M. R. Khazaei, M.-P. Girouard, R. Alchini, S. O. Tone, T. Shimada, S. Bechstedt, M. Cowan, D. Guillet, P. W. Wiseman, G. Brouhard, J. F. Cloutier, A. E. Fournier. Collapsin response mediator protein 4 regulates growth cone dynamics through the actin and microtubule cytoskeleton. J. Biol. Chem. 289: 30133-30143 (2014)

  • L.E. Lorenzo, A. G. Godin, F. Wang, M. St-Louis, S. Carbonetto, P. W. Wiseman, A. Ribeiro-da-Silva and Y. De Koninck. Gephyrin Clusters Are Absent from Small Diameter Primary Afferent Terminals Despite the Presence of GABAA Receptors. J. Neurosci. 34: 8300-8317 (2014)

  • K. E. Bujold, J. Fakhoury, T. G. W. Edwardson, K. M. M. Carneiro, J. N. Briard, A. G. Godin, L. Amrein, G. D. Hamblin, L. C. Panasci, P. W. Wiseman and H. F. Sleiman. Sequence-responsive unzipping DNA cubes with tunable cellular uptake profiles. Chem. Sci. 5: 2449-2455 (2014)

  • N. Jowett, W. Wöllmer, R. Reimer, J. Zustin, U. Schumacher, P. Wiseman, A. Mlynarek, A. Böttcher, C. Dalchow, B. Lörincz, R. Knecht, and R. J. D. Miller. Bone Ablation without Thermal or Acoustic Mechanical Injury via a Novel Picosecond Infrared Laser (PIRL). JAMA Otolaryngology - Head & Neck Surgery 150: 385-393 (2014)

  • H. B. Brandao, H. Sangji, E. Pandzic, S. Bechstedt, G. J. Brouhard, P. W. Wiseman. Measuring ligand-receptor binding kinetics and dynamics using k-space image correlation spectroscopy. Methods 66: 273-282 (2014)
    Invited Paper: Special issue on Advanced Light Microscopy Imaging in Biological Sciences.

    F1000Prime Recommendation


  • E. C. Arnspang, J. S. Koffman, S. Marlar, P. W. Wiseman, L. N. Nejsum. Easy measurement of diffusion coefficients of EGFP-tagged plasma membrane proteins using a MatLab code for k-space Image Correlation Spectroscopy. JoVE 87 (2014)

  • E. C. Arnspang, J. Schwartzentruber, M. P. Clausen, C. Lyngsø, J. L. Hansen, P. W. Wiseman, B. C. Lagerholm. Bridging the gap between single molecule and ensemble methods for measuring lateral dynamics in the plasma membrane. PLoS ONE 8(12): e78096 (2013)

  • P. W. Wiseman. Fluctuation Imaging Spiced Up with a Piece of PIE. Biophys. J. 105: 831 (2013)

  • A. Barbeau, J. L. Swift, A. G. Godin, Y. De Koninck, P.W. Wiseman, J.-M. Beaulieu. Spatial Intensity Distribution Analysis (SpiDA), a new tool for receptor tyrosine kinase activation and transactivation quantification. Methods in Cell Bio. 117: 1-19 (2013)

  • L. Potvin-Trottier, L. Chen, A. R. Horwitz, P. W. Wiseman. A nu-space for ICS: characterization and application to measure protein transport in live cells. New J. Phys. 15: 085006 (2013). Focus on Soft Mesoscopics: Physics for Biology at a Mesoscopic Scale

  • K. Hossein Heris, A. K. Miri, U. Tripathy, P. W. Wiseman, L. Mongeau. Microstructural Characterization of Vocal Folds toward a Strain-Energy Formulation. Acta Biomater. 9(8): 7957-7967 (2013)

  • U. Tripathy, M. Giguère-Bisson, M. H. Sangji, M.-J. Bellemare, D. S. Bohle, E. George, P. W. Wiseman. Optimization of the third harmonic generation response for imaging detection of the malaria pigment hemozoin. Anal. Bioanal. Chem. 405: 5431-5440 (2013)

  • P. W. Wiseman. Image correlation spectroscopy: mapping correlations in space, time, and reciprocal space. Meth. Enzymol. 518: 245-267 (2013)

  • B. Rappaz, P. W. Wiseman. Image Correlation Spectroscopy for measurements of particle densities and colocalization. Curr. Protoc. Cell Bio. 4.27.1-4.27.15 (2013)

  • N. Jowett, W. Woellmer, A. M. Mlynarek, P. Wiseman, B. Segal, K. Franjic, P. Kroetz, A. Boettcher, R. Knecht, R. J. D. Miller. Heat generation during ablation of porcine skin with Er:YAG laser versus a novel picosecond infrared laser (PIRL). JAMA Otolaryngology — Head & Neck Surgery 139(8): 828-833 (2013)

  • A. Barbeau, A. G. Godin, J. L. Swift, Y. De Koninck, P. W. Wiseman, and J.-M.Beaulieu. Quantification of receptor tyrosine kinase activation and transactivation by G Protein Coupled receptors using Spatial Intensity Distribution Analysis (SpIDA). Meth. Enzymol. 522: 109-131 (2013)

  • T. Toplak, E. Pandzic, L. Chen, M. Vicente-Manzanares, A. R. Horwitz, P. W. Wiseman. STICCS Reveals Matrix-Dependent Adhesion Slipping and Gripping in Migrating Cells. Biophys. J. 103(8):1672-1682 (2012)

  • L. Chen, M. Vicente-Manzanares, L. Potvin-Trottier, P. W. Wiseman, A. R. Horwitz. The integrin-ligand interaction regulates adhesion and migration through a molecular clutch. PLoS ONE 7(7):1-15 (2012)

  • M. Sergeev, A. G. Godin, L. Kao, N. Abuladze, P. W. Wiseman, I. Kurtz. Determination of Membrane Protein Transporter Oligomerization in Native Tissue Using Spatial Fluorescence Intensity Fluctuation Analysis. PLoS ONE 7(4):1-11 (2012)

  • M. Sergeev, J. L. Swift, A. G. Godin, P. W. Wiseman. Ligand-induced clustering of EGF receptors : A quantitative study by fluorescence image moment analysis. Biophys. Chem. 161:50-53 (2012)

  • A. K. Miri, U. Tripathy, L. Mongeau, P. W. Wiseman. Nonlinear Laser Scanning Microscopy of Human Vocal Folds. The Laryngoscope 122(2):356-363 (2012)

  • N. Durisic, A. Godin, D. Walters, P. Grutter, P. W. Wiseman, C. Heyes. Probing the “dark” fraction of core-shell quantum dots by ensemble and single particle pH-dependent spectroscopy. ACS Nano 5(11):9062-9073 (2011)

  • S. Boyle, D. Kolin, J. G. Bieler, J. P. Schneck , P. W. Wiseman, M. Edidin. Quantum dot fluorescence characterizes the nanoscale organization of T cell receptors for antigen. Biophys. J. 101:L57-L59 (2011)

  • H. Balghi, R. Robert, B. Rappaz, X. Zhang, A. Wohlhuter-Haddad, A. Evagelidis, Y. Luo, J. Goepp, P. Ferraro, P. Roméo, M. Trebak, P. W. Wiseman, D. Y. Thomas, J. W. Hanrahan. Enhanced Ca2+ entry due to Orai1 plasma membrane insertion increases IL-8 secretion by cystic fibrosis airways. FASEB J. 25(12):4274-4291 (2011)

  • B. Mesnage, S. Gaillard, A. Godin, J.-L. Rodeau, M. Hammer, J. von Engelhardt, P. W. Wiseman, Y. De Koninck, R. Schlichter, M. Cordero-Erausquin. Morphological and functional characterization of cholinergic interneurons in the dorsal horn of the mouse spinal cord. Journal of Comparative Neurology 519:3139-3158 (2011)

  • J. L. Swift, A. G. Godin, K. Doré, L. Freland, N. Bouchard, C. Nimmo, M. Sergeev, Y. De Koninck, P. W. Wiseman, J. M. Beaulieu. Quantification of receptor tyrosine kinase transactivation through direct dimerization and surface density measurements in single cells. Proc. Natl. Acad. Sci. U.S.A. 108(17): 7016-7021 (2011)

  • A. G. Godin, S. Costantino, L. E. Lorenzo, J. L. Swift, M. Sergeev, A. Ribeiro-da-Silva, Y. De Koninck, P. W. Wiseman. Revealing protein oligomerization and densities in situ using spatial intensity distribution analysis. Proc. Natl. Acad. Sci. U.S.A. 108(17): 7010-7015(2011)

  • V. Baumgärtel, S. Ivanchenko, A. Dupont, M. Sergeev, P. W. Wiseman, H-G Kräusslich, C. Bräuchle, B. Müller, D. C. Lamb Dynamics of HIV budding site interactions with an ESCRT component visualized in live-cells. Nature Cell Biology 13:469–474 (2011)

  • J. L. Swift, M. Sergeev, P.W. Wiseman. Fluorescence microscopy investigations of ligand propagation and accessibility under adherent cells. Biointerfaces 5(4): (2010)

  • P. Opazo, S. Labrecque, C. M. Tigaret, A. Frouin, P. W. Wiseman, P. De Koninck, D. Choquet. CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of Stargazin. Neuron 67(2):239-252 (2010)

  • J. M. Belisle, T. Zigras, S. Costantino, R. Cartier, J. Butany, P. W. Wiseman, R. L. Leask. Second Harmonic Generation Microscopy to Investigate Collagen Configuration: A Pericarditis Case Study. Cardiovascular Pathology 19(4):e125-e128 (2009)

  • N. Durisic, P. W. Wiseman, P. Grutter, C. Heyes. A common mechanism underlies the dark fraction formation and fluorescence blinking of quantum dots. ACS Nano 3(5): 1167-1175 (2009)

  • K. G. Heinze, S. Costantino, P. De Koninck, P. W. Wiseman. Beyond photobleaching, laser illumination unbinds fluorescent proteins. J Phys Chem B. 113(15): 5225-33(2009)

  • M. A. Digman, P. W. Wiseman, C. Choi, A. R. Horwitz and E. Gratton. Stoichiometry of molecular complexes at adhesions in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (7): 2170-2175 (2009)

  • M. A. Digman, P. W. Wiseman, A. R. Horwitz, E. Gratton. Detecting Protein Complexes in Living Cells from Laser Scanning Confocal Image Sequences by the Cross Correlation Raster Image Spectroscopy Method. Biophys. J. 96 (2): 707-716 (2009)
    Faculty of 1000 Biology Selection

  • J. M. Bélisle, J. P. Correia, P. W. Wiseman, T. E. Kennedy, S. Costantino. Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP. Lab on a Chip. 147: DOI: 10.1039/b813897d (2008) Highlighted in Chemical Technology October 30, 2008

  • J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, A. Geitmann. Magnitude and direction of vesicle dynamics in growing pollen tubes using spatiotemporal image correlation spectroscopy (STICS) and fluorescence recovery after photobleaching (FRAP). Plant Physiology. 147: 1646-1658 (2008)

  • P. J. Darlington, C. Podjaski, K. E. Horn, S. Costantino, M. Blain, P. Saikali, Z. Chen, K. A. Baker, J. Newcombe, M. Freedman, P. W. Wiseman, A. Bar-Or, T. E. Kennedy, J. P. Antel. Innate Immune-mediated Neuronal Injury Consequent to Loss of Astrocytes. Journal of Neuropathology and Experimental Neurology. 67(6): 590-599 (2008)

  • J. W. D. Comeau, D. L. Kolin, P. W. Wiseman. Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy. Molecular Biosystems. 4: 672 - 685 (2008)
    Invited paper "New Emerging Investigators special issue"


  • A. I. Bachir, D. L. Kolin, K. G. Heinze, B. Hebert, P. W. Wiseman. A guide to accurate measurement of diffusion using fluorescence correlation techniques with blinking quantum dot nanoparticle labels. J. Chem. Phys. 128: 225105-1-225105-11 (2008)

  • S. Costantino, C. B. Kent, A. G. Godin, T. E. Kennedy, P. W. Wiseman, A. E. Fournier. Semi-automated quantification of filopodial dynamics. Journal of Neuroscience Methods. 171: 165-173 (2008)

  • J. M. Belisle, S. Costantino, M. L. Leimanis, M.-J. Bellemare, D. S. Bohle, E. Georges, P. W. Wiseman. Sensitive Detection of Malaria Infection by Third Harmonic Generation Imaging. Biophys. J.: Biophysical Letter 94:L26-L28 (2008) Highlighted in Biophotonics International, February 2008; Highlighted in BioOptics World March 2008; Highlighted in MalariaWorld; Highlighted in Analytical Chemistry Online News October 30, 2008.

  • R. Khatchadourian, A. I. Bachir, S. J. Clarke, C. D. Heyes, P. W. Wiseman, J. L. Nadeau. Fluorescence intensity and intermittency as tools for tracking. Biomed.Biotech. 70145:1-10 (2008)

  • S. Costantino, C. G. McQuinn, T. E. Kennedy, P. W. Wiseman. Fabrication of protein gradients for cell culture using a miniature squeegee. Journal of Biochemical and Biophysical Methods 70: 1192-1195 (2008)

  • P. T. Yam, C. A. Wilson, L. Ji, B. Hebert, E. L. Barnhart, N. A. Dye, P. W. Wiseman, G. Danuser, J. A. Theriot. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. Journal of Cell Biology 178:1207-1221 (2007)

  • D. L. Kolin, P. W. Wiseman. Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently-labeled macromolecules in cells. Cell Biochem. Biophys. 49: 141-164 (2007)

  • N. Durisic, A. I. Bachir, D. L. Kolin, B. Hebert, B. C. Lagerholm, P. Grutter, P. W. Wiseman. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules. Biophys. J. 93: 1338-1346 (2007)

  • D. Fessart, M. Simaan, B. Zimmerman, J. Comeau, F. F. Hamdan, P. W. Wiseman, M. Bouvier, S. A. Laporte. SRC-dependent phosphorylation of beta2-adaptin dissociated the beta-arrestin-AP-2 complex. Journal of Cell Science 120: 1723-1732 (2007)

  • J. W. D. Comeau, S. Costantino, P. W. Wiseman. Measuring colocalization within fluorescence microscopy images. Biophotonics International 14 (3):42-45 (2007)

  • C. M. Brown, B. Hebert, D. L. Kolin, J. Zareno, L. Whitmore, A. R. Horwitz, P. W. Wiseman. Probing the integrin-actin linkage using high-resolution protein velocity mapping. Journal of Cell Science 119:5204-5214 (2006)
    Faculty of 1000 Biology Selection



  • I. R. Bates, P. W. Wiseman, J. W. Hanrahan. Investigating membrane protein dynamics in living cells. Biochemistry & Cell Biology 84:825-831 (2006) Invited Review

  • J. W. D. Comeau, S. Costantino, P. W. Wiseman. A Guide to Accurate Fluorescence Microscopy Colocalization Measurements. Biophys. J. 91:4611-4622 (2006)

  • M. Sergeev, S. Costantino, P. W. Wiseman. Measurement of Monomer-Oligomer Distributions via Fluorescence Moment Image Analysis." Biophys. J. 91:3884-3896 (2006)

  • D. L. Kolin, D. Ronis, P. W. Wiseman. k-Space image correlation spectroscopy (kICS): A method for accurate transport measurements independent of fluorophore photophysics. Biophys. J. 91: 3061-3075 (2006)

  • I. R. Bates, B. Hebert, Y. Luo, J. Liao, A. I. Bachir, D. L. Kolin, P. W. Wiseman, J. W. Hanrahan. Membrane lateral diffusion and capture of CFTR within transient confinement zones. Biophys. J. 91(3):1046-1058 (2006)
    Faculty of 1000 Biology Selection

  • A. Bachir, N. Durisic, B. Hebert, P. Grutter, P. W. Wiseman. Characterization of Blinking Dynamics in Quantum Dot Ensembles using Image Correlation Spectroscopy. J. Appl. Phys. 99: 064503-1-064503-7 (2006). Article selected for March 27, 2006 issue of Virtual Journal of Nanoscale Science & Technology and also selected for the April 1, 2006 issue of Virtual Journal of Biological Physics Research (editorial selection of the most important research papers of the month).

  • D. L. Kolin, S. Costantino, and P. W. Wiseman. Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy. Biophy.J. 90(2):628-639 (2006).

  • S. Costantino, K. G. Heinze, O. E. Martinez, P. De Koninck, P. W. Wiseman. Two-photon fluorescent micro-lithography for live cell imaging Microscopy Research and Technique 68 : 272-276 (2005)

  • S. Costantino, J. W. D. Comeau, D. L. Kolin, P. W. Wiseman. Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy Biophys. J. 89 : 1251-1260 (2005).

  • B. Hebert, S. E. Hulme, P. W. Wiseman. Membrane protein dynamics measured by two-photon ring correlation spectroscopy: theory and application to living cells. Proceedings of SPIE 5700: 109-117 (2005). Invited Paper

  • M. A. Digman, C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, E. Gratton, Measuring fast dynamics in solutions and cells with a laser scanning microscope, Biophys. J. 89 : 1317-1327 (2005)
    Faculty of 1000 Biology Selection

  • M.A. Digman, P. Sengupta, P.W. Wiseman, C.M. Brown, A.R. Horwitz, E. Gratton. Fluctuation correlation spectroscopy with laser scanning microscope: exploiting the hidden time structure. Biophys. J. 88 : L33-36 (2005).

  • B. Hebert, S. Constantino, P. W. Wiseman. Spatio-temporal Image Correlation Spectroscopy (SITCS): Theory, verification and application to protein velocity mapping in living CHO cells. Biophys. J. 88, 3601-3614 (2005).

  • P. W. Wiseman, C.M. Brown, D.J. Webb, B. Hebert, N.L.Johnson, J.A. Squier, M.H. Ellisman, A.R. Horwitz. Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy, J. Cell Sci. 117, 5521-5534 (2004) Highlighted in Nature Reviews Molecular Cell Biology.
    Faculty of 1000 Biology Selection


  • J. V. Rocheleau, P. W. Wiseman, N. O. Petersen, Isolation of bright aggregate fluctuations in a multipopulation image correlation spectroscopy system using intensity subtraction, Biophys. J. 84, 4011-4022 (2003).

  • P. W. Wiseman, J. A. Squier, Measurement of cell surface protein dynamics by two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4633, 74-83(2002).

  • P. W. Wiseman, J. A. Squier, Live cell studies of adhesion receptors by two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4620, 54-61 (2002).

  • P. W. Wiseman, F. Capani, J. A. Squier, and M. E. Martone, Counting dendritic spines in cerebellar tissue slice by image correlation spectroscopy analysis, J. Microsc. 205, 177-186 (2002).

  • P. W. Wiseman and J. A. Squier, Two-photon image correlation spectroscopy and image cross-correlation spectroscopy, Proc. SPIE 4262, 279-286 (2001).

  • D. N. Fittinghoff, P. W. Wiseman, J. A. Squier, Widefield multiphoton and temporally decorrelated multifocal multiphoton microscopy, Opt. Exp. 7, 273-279 (2000).

  • P. W. Wiseman, J. A. Squier, M. H. Ellisman, and K. R. Wilson, Two-photon video rate image correlation spectroscopy (ICS) and image cross-correlation spectroscopy (ICCS), J. Microscopy 200, 14-25 (2000).

  • P. W. Wiseman, J. A. Squier, K. R. Wilson, Dynamic image correlation spectroscopy (ICS) and two-color image cross-correlation spectroscopy (ICCS): concepts and application, Proc. SPIE 3919, 14-20 (2000).

  • A. C. Millard, P. W. Wiseman, D. N. Fittinghoff, K. R. Wilson, J. A. Squier, and M. Müller, Third harmonic generation microscopy using a compact, femtosecond fiber laser source, App. Opt. 38, 7393-7397 (1999).

  • P. W. Wiseman and N. O. Petersen, Image correlation spectroscopy (ICS) II: optimization for ultrasensitive detection of pre-existing PDGF-b receptor oligomers on intact cells, Biophys. J. 76, 963-977 (1999).

  • N. O. Petersen, C. M. Brown, M. Srivastava, J. Rocheleau, P. W. Wiseman, Analysis of membrane protein cluster densities and sizes by in situ image correlation spectroscopy, Faraday Discuss. 111, 289-305 (1998).

  • P. W. Wiseman, P. Höddelius, N. O. Petersen, and K.-E. Magnusson, Aggregation of PDGF-b receptors in human skin fibroblasts: characterization by image correlation spectroscopy (ICS), FEBS Lett. 401, 43-48 (1997).

  • N. O. Petersen, P. L. Höddelius, P. W. Wiseman, O. Seger, and K.-E. Magnusson, Quantitation of membrane receptor distributions by image correlation spectroscopy: concept and application, Biophys. J. 65, 1135-1146 (1993).

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